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primary mouse embryo fibroblasts (mefs)  (Janvier Labs)

 
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    Janvier Labs primary mouse embryo fibroblasts (mefs)
    Primary Mouse Embryo Fibroblasts (Mefs), supplied by Janvier Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+mouse+embryo+fibroblasts+(mefs)/primary+mouse+embryo+fibroblasts++mefs+/bio_rxiv__695684-35-0-16
    Average 90 stars, based on 1 article reviews
    primary mouse embryo fibroblasts (mefs) - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Homeoprotein neuroprotection of embryonic neuronal cells
    Article Snippet: Primary mouse embryo fibroblasts (MEFs) were isolated from the skin of 11 dpc Swiss mouse embryos (Janvier) according to .



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    Development and characterization of a gliomagenic recombinant lentiviral vector encoding shCdkn2a-PDGFB-T2A-CreERT2. (A) Vector design and (B) expression of PDGFB and CreERT2 from the vector in 293FT cells. (C) Quantitative real-time PCR analysis of Cdkn2a expression in primary mouse fibroblasts infected with the shCdkn2a virus compared with a control shRNA (2 multiplicity of infection). Mean (C) ± 1 SD of 3 independent experiments are shown. **P < 0.01.

    Journal: Neuro-Oncology

    Article Title: A recombinant lentiviral PDGF-driven mouse model of proneural glioblastoma

    doi: 10.1093/neuonc/nox129

    Figure Lengend Snippet: Development and characterization of a gliomagenic recombinant lentiviral vector encoding shCdkn2a-PDGFB-T2A-CreERT2. (A) Vector design and (B) expression of PDGFB and CreERT2 from the vector in 293FT cells. (C) Quantitative real-time PCR analysis of Cdkn2a expression in primary mouse fibroblasts infected with the shCdkn2a virus compared with a control shRNA (2 multiplicity of infection). Mean (C) ± 1 SD of 3 independent experiments are shown. **P < 0.01.

    Article Snippet: Cell Culture We grew 293FT (ThermoFisher Scientific), primary mouse embryo fibroblast (MEF; isolated at e12.5 from C57BL/6J mice [Jax Stock No: 000664]), and U251 (originally obtained from American Type Culture Collection) cells on tissue culture dishes in growth media consisting of Dulbecco’s modified Eagle’s medium with L-glutamine (Corning) supplemented with 1% penicillin/streptomycin (Corning) and 10% fetal bovine serum (Corning).

    Techniques: Recombinant, Plasmid Preparation, Expressing, Real-time Polymerase Chain Reaction, Infection, Virus, Control, shRNA